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This study is designed to demonstrate and compare the efficacy of alcohol-based scrub-free solutions versus iodine鈥揵ased scrub solutions as presurgical antiseptic solutions on reducing bacterial hand loads.

First, antiseptic solutions were measured using a measuring cup to yield uniform results of both solutions. For the respondents who used 10% Povidone-iodine, respondents started the proper surgical hand scrubbing by using approximately 6 ml of antiseptic solution while those who used an alcohol based scrub-free solution; they were required to perform hand-washing before applying the said solution.

All 30 subjects completed the surgical hand scrubbing using the assigned solutions: 10% Povidone-iodine and the alcohol scrub-free solution. The colony forming unit before the application of the 10% Povidone-iodine solution measured in 24 hours was 10.84X103 and was reduced to 0.70x103 after the application of 10% Povidone-iodine resulting to a mean difference of 10.14x103. Using alcohol based scrub-free solution as presurgical solution in 15 randomized respondents, the study had shown that before the application of the solution, the average colony forming unit measured in 24 was 13.36x103 and was reduced to 0.61x103 after the application of alcohol scrub-free solution resulting to a mean difference of 12.74x103. The results will tell us that there is a significant difference before and after the application of the two solutions. Using the t-test with the results given, there is no significant mean difference in the CFU count between the two applications. The average CFU after 10% Povidone-iodine application is 0.70X103 and the average CFU after the Alcohol application is 0.61X103. Their mean difference is only 1.93X103. To test that the two means are statistically different from each other, t-test was employed. The computed t-value is 1.28 and its associated p-value of 0.21 which is greater than the level of significance 0.050 indicates that the null hypothesis is accepted. Thus, there was no significant mean difference in the CFU count between the two applications. This study suggested that with no significant mean difference between the two applications, the hospital can either use either of the two solutions since they are equally potent in reducing microbial loads. Future studies should expound in identifying specifically what kind of bacteria is found on hands before and after scrubbing with the 10% Povidone-iodine and alcohol-based scrubbing solution. Also, to increase the number of respondents for each scrubbing solution making sure that the allotted time is enough to accommodate all of the respondents. And, finally to include in the study the use of other pre-surgical scrubbing solutions such as, Chlorhexidine gluconate, Parachlorometaxylenol, Triclosan making the study more comprehensive and reliable base on wide range of results as basis of choosing or comparing which scrubbing solution best lessens the number of microbial loads on hands prior to an operation.