| Title |
High-throughput sequencing-based analysis of bacterial communities associated with Barbour's seahorses (Hippocampus barbouri) from Surigao del Norte, Philippines |
|
Authors |
Rose Chinly Mae H. Ortega, Sharon Rose M. Tabugo, Joey Genevieve T. Martinez, Chinee S. Padasas, Marilen P. Balolong, José L. Balcázar |
|
Publication date |
2021 |
|
Journal |
Letters in Applied Microbiology |
| Pages |
1-6 |
| Publisher |
WILEY , 111 RIVER ST, HOBOKEN, USA, NJ, 07030-5774 |
| Abstract |
This study identified the bacterial community composition associated with
Barbour’s seahorses (Hippocampus barbouri). Seahorses and samples from the
surrounding environment (sediment and water) were collected from Cantiasay
Island in Surigao del Norte, Philippines. Genomic DNA was isolated from
these samples, and the V1–V3 region of the 16S rRNA gene was amplified and
sequenced on the Illumina MiSeq platform. There were 929 803 sequence reads
corresponding to 859 operational taxonomic units (OTUs), which were
obtained from the six genomic libraries. The results showed that the most
abundant OTUs were affiliated to the phylum Proteobacteria, particularly those
belonging to the genera Shewanella, Pseudomonas, Acinetobacter and
Aeromonas, which were found to be dominant in seahorse gut samples. The
only exception was for the skin of male seahorses, which was dominated by
members belonging to the genus Bacillus (phylum Firmicutes). These findings
on bacterial community structure and composition may shed light on
therapeutic claims of Barbour’s seahorses and help in the conservation of this
species. |
| Index terms / Keywords |
bacterial community composition, Hippocampus barbouri, seahorses. |
| DOI |
doi:10.1111/lam.13511 |
| URL |
|
Mindanao State
University - Iligan Institute of Technology